pJ609 Flag-Sumo1
(Plasmid #49046)

• Purpose: Mammalian expression of N-terminally tagged Sumo1
• Depositing Lab: Lianna Swanson
• Publication: Addgene's unpublished plasmids (unpublished)

Backbone

• Vector backbone: pJexpress609
• Backbone manufacturer: DNA2.0
• Total vector size (bp): 5127
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO1
• Species: H. sapiens (human)
• Insert Size (bp): 357
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • DNA 2.0 Limited Use Label License
• Industry Terms:
  • Not Available to Industry