pcDXNSM3
(Plasmid #49028)

• Purpose: (Empty Backbone) FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site
• Depositing Labs: Raimund Dutzler, Eric Geertsma
• Publication: Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5855
• Modifications to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
• Vector type: Mammalian Expression
• Promoter: CMV
• Tags / Fusion Proteins:
  • streptavidin binding peptide (SBP) (N terminal on backbone)
  • MYC (N terminal on backbone)
  • 3C protease site (PreScission site) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.