C321.ΔA.exp
(Bacterial strain #49018)

• Purpose: This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C
• Depositing Lab: George Church
• Publication: Lajoie et al Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459.

Backbone

• Vector backbone: E. coli genome
• Total vector size (bp): 4633801
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): this strain
• Growth instructions: This strain has mutS+ (reduced mutation rate compared to C321.deltaA) and lambda- (growth can be performed at 37C). This strain must be supplemented with d-biotin when grown in minimal media.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
• Alt name: C321.deltaA.exp
• Alt name: C321.ΔA.exp
• Species: E. coli
• Insert Size (bp): 4633801
• Mutation: See genbank file
• GenBank ID:

Resource Information

• Articles Citing this Plasmid:
  • 29 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA

This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.

The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).