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1287 p3E-mut let-7 etv2 3’ UTR
(Plasmid #49011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRP2RP3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2622
  • Total vector size (bp) 3672
  • Vector type
    gateway 3' Entry

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ets variant gene 2
  • Alt name
    etv2
  • Species
    D. rerio (zebrafish)
  • Mutation
    Mutated the Let7 miRNA binding sites
  • Entrez Gene
    etsrp (a.k.a. ets1b, etv2, grc, wu:fc49b07, zgc:153819)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1287 p3E-mut let-7 etv2 3’ UTR was a gift from Nathan Lawson (Addgene plasmid # 49011 ; http://n2t.net/addgene:49011 ; RRID:Addgene_49011)

  • For your References section:

    Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310
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