800 pME-Bozozok
(Plasmid
#49008)
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Purposefull length coding sequence for zebrafish bozozok gene in gateway middle entry vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepdonr221
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 2547
- Total vector size (bp) 3127
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Vector typegateway middle entry
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedharma
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Alt namedharma
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Insert Size (bp)580
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Entrez Genedharma (a.k.a. boz, dha, nwk)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13F
- 3′ sequencing primer m13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
800 pME-Bozozok was a gift from Nathan Lawson (Addgene plasmid # 49008 ; http://n2t.net/addgene:49008 ; RRID:Addgene_49008) -
For your References section:
Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N. Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. 10.1016/j.ydbio.2013.08.028 PubMed 24036310
