C321
(Bacterial strain #48999)

• Purpose: Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)
• Depositing Lab: George Church
• Publication: Lajoie et al Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459.

Backbone

• Vector backbone: E. coli genome
• Total vector size (bp): 4644726
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): this strain
• Growth instructions: Growth above 34 C is not recommended, since growth at elevated temperatures leads to leaky expression of toxic proteins from the lambda prophage. A lambda- version will be deposited at addgene soon.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Recoded E. coli genome with all instances of the UAG codon removed, but wild type UAG termination function is not changed
• Species: E. coli
• Mutation: See genbank file of C321.DA
• GenBank ID:

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli MG1655 delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta mutS::zeoR; all 321 UAG codons changed to UAA

This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.

Sequence is similar to C321.DA (NCBI accession # NCBI CP006698), except that RF1 is not deleted.