pDONR221-Hyg
(Plasmid #48994)

• Purpose: Gateway adapted transfer of Hygromycin resistance gene
• Depositing Lab: Rick Tarleton
• Publication: Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Life Technologies (Invitrogen)
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 3500
• Vector type: Gateway attB1 & attB2
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hygromycin B phosphotransferase
• Alt name: Hygromycin B resistance gene
• Insert Size (bp): 1029

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 for
• 3′ sequencing primer: M13 rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.