pDONR221-Neo
(Plasmid #48993)

• Purpose: Gateway adapted transfer of Neomycin resistance gene
• Depositing Lab: Rick Tarleton
• Publication: Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10.1186/1471-2180-9-90.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Life Technologies (Invitrogen)
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 3300
• Vector type: Gateway attB1 & attB2
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aminoglycoside 3' phosphotransferase
• Alt name: Neomycin resistance gene
• Insert Size (bp): 804

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 for
• 3′ sequencing primer: M13 rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.