pBEG R2-iPuro-L3 #BGV205
(Plasmid #48987)

• Purpose: Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
• Depositing Lab: David Dankort
• Publication: Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279.

Backbone

• Vector backbone: pBEG R2+xL3 (Addgene plasmid 48952)
• Backbone manufacturer: Dankort Lab
• Total vector size (bp): 3480
• Vector type: Gateway cloning vector
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: puromycin resistance
• Alt name: N-acetyl-transferase
• Promoter: NA
• Tag / Fusion Protein:
  • IRES (weak) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.