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Purpose(Empty Backbone) 3rd generation Lentiviral vector with bi-cistronic expression of the blue fluorescent protein AmCyan and the gene of interest.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUltra
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Backbone manufacturerMalcolm Moore lab
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Modifications to backboneeGFP was replaced with the blue fluorescent protein AmCyan from Anemonia majano.
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Vector typeMammalian Expression, Lentiviral
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Tag
/ Fusion Protein
- AmCyan (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer WPRE-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AmCyan1 excitation and emission maxima are 458 and 489 nm, respectively
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUltra-Smurf was a gift from Malcolm Moore (Addgene plasmid # 48974 ; http://n2t.net/addgene:48974 ; RRID:Addgene_48974)
