pET21 Catalytic Domain of MALT1
(Plasmid #48970)

• Purpose: Expresses catalytic domain of MALT1 in e.coli AA 329-566
• Depositing Lab: Guy Salvesen
• Publication: Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6199
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MALT1
• Species: H. sapiens (human)
• Insert Size (bp): 756
• Mutation: Contains AA 329-566
• Entrez Gene: MALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcorI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry