pcDNA3 HA-Sumo1 Q94P
(Plasmid #48965)

• Purpose: Expresses HA SUMO1 Q94P mutant in mammalian cells
• Depositing Lab: Guy Salvesen
• Publication: Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 327
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO1
• Species: H. sapiens (human)
• Insert Size (bp): 327
• Mutation: Mutated Q94P
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: T7
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry