pBEG R1-ChlorR-R4 #BGV049
(Plasmid #48943)

• Purpose: (Empty Backbone) Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors
• Depositing Lab: David Dankort
• Publication: Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279.

Backbone

• Vector backbone: pOK1/2
• Backbone manufacturer: Messing Lab, PMID 1905257
• Backbone size (bp): 3766
• Modifications to backbone: The chloramphenicol selection cassette was PCR amplified from a lab Gateway destination vector (gQxiPuro, unpublished plasmid) using the following forward (5’-CACCTCTAGACTCGAGATTAGGCACCCCAGGCTTTACAC) and reverse (5’-ATATGAATTCGTCGACCTGCAGACTGGCTGTG) primers and cloned into the XbaI site of pOK1/2 B giving pOK1/2 B (ChlorR). Next, the attR1 site from pUC57 fragment A was cloned into this vector using BglII/NotI giving pBEG R1-ChlorR-R4. Finally, the ccdB-ChloroR cassette from gQxiPuro was cloned into pBEG R1-ChloroR-R4 vectors with NotI/SalI.
• Vector type: Gateway cloning vector
• Promoter: na

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.