pTD103aiiA(Cm) -R
(Plasmid
#48888)
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PurposeNegative feedback plasmid from oscillator without luxR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48888 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepZ vector
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaiiA
- Promoter pLux
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Tag
/ Fusion Protein
- ssrA tag (AANDENYALAA) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTD103aiiA(Cm) -R was a gift from Jeff Hasty (Addgene plasmid # 48888 ; http://n2t.net/addgene:48888 ; RRID:Addgene_48888) -
For your References section:
A sensing array of radically coupled genetic 'biopixels'. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. 10.1038/nature10722 PubMed 22178928
Map uploaded by the depositor.
