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PurposeNegative feedback plasmid from oscillator
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepZ vector
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacI with ssrA tag
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Insert Size (bp)1122
- Promoter Para/lac
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Tag
/ Fusion Protein
- ssrA (TSAANDENYALAA) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJS169 was a gift from Jeff Hasty (Addgene plasmid # 48882 ; http://n2t.net/addgene:48882 ; RRID:Addgene_48882) -
For your References section:
A fast, robust and tunable synthetic gene oscillator. Stricker J, Cookson S, Bennett MR, Mather WH, Tsimring LS, Hasty J. Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29. 10.1038/nature07389 PubMed 18971928
Map uploaded by the depositor.
