pEx_Hsp70::attBmut
(Plasmid
#48878)
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Purpose(Empty Backbone) PhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTRdTopo
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Backbone manufacturerLife Technologies
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEx_Hsp70::attBmut was a gift from Joachim Wittbrodt (Addgene plasmid # 48878 ; http://n2t.net/addgene:48878 ; RRID:Addgene_48878) -
For your References section:
Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase. Kirchmaier S, Hockendorf B, Moller EK, Bornhorst D, Spitz F, Wittbrodt J. Development. 2013 Sep 18. 10.1242/dev.096081 PubMed 24048591