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Addgene

pJSM103
(Plasmid #48872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmsg288
  • Total vector size (bp) 13082
  • Modifications to backbone
    Insert into LacZ gene at EcorV.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Kanamycin 40mcg/ml
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    split lacZ

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJSM103 was a gift from Michael Glickman (Addgene plasmid # 48872 ; http://n2t.net/addgene:48872 ; RRID:Addgene_48872)

  • For your References section:

    Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D. Zhu H, Bhattarai H, Yan HG, Shuman S, Glickman MS. Biochemistry. 2012 Dec 21;51(51):10147-58. doi: 10.1021/bi301202e. Epub 2012 Dec 11. 10.1021/bi301202e PubMed 23198659
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