pGGI000
(Plasmid #48863)

• Purpose: (Empty Backbone) Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.
• Depositing Lab: Jan Lohmann
• Publication: Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043.

Backbone

• Vector backbone: pUC19
• Backbone size (bp): 2686
• Vector type: Golden Gate Compatible cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp