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Addgene

pGGF000
(Plasmid #48861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size (bp) 2686
  • Vector type
    Golden Gate Compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF000 was a gift from Jan Lohmann (Addgene plasmid # 48861 ; http://n2t.net/addgene:48861 ; RRID:Addgene_48861)

  • For your References section:

    GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629
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