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Addgene

pGGF004
(Plasmid #48845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Golden Gate compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p35S::D-AlanineR::t35S
  • Alt name
    D-alanine resistance cassette
  • Species
    Synthetic
  • Insert Size (bp)
    2183
  • Mutation
    internal BsaI recognition site in 35S promoter removed by substitution

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed for Golden Gate cloning using the BsaI enzyme.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
D-alanine resistance cassette with 35S promoter and terminator = 2252-4434bp
BsaI site #2 = 4435-4445bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF004 was a gift from Jan Lohmann (Addgene plasmid # 48845 ; http://n2t.net/addgene:48845 ; RRID:Addgene_48845)

  • For your References section:

    GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629
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