Skip to main content
Addgene

pGGF003
(Plasmid #48844)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48844 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Golden Gate compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pMAS::D-AlanineR::tMAS
  • Alt name
    D-alanine resistance cassette
  • Species
    Synthetic
  • Insert Size (bp)
    1758

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed for Golden Gate cloning using the BsaI enzyme.

Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
D-alanine resistance cassette with MAS promoter and terminator = 2252-4009bp
BsaI site #2 = 4010-4020bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF003 was a gift from Jan Lohmann (Addgene plasmid # 48844 ; http://n2t.net/addgene:48844 ; RRID:Addgene_48844)

  • For your References section:

    GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed 24376629
Included in kit:
Related items:
Commonly requested with: