pcDNA3.1-nAChr-Alpha6
(Plasmid #48812)

• Purpose: this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
• Depositing Lab: Henry Lester
• Publication: Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.

Backbone

• Vector backbone: pcDNA3.1 Zeo
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: nAChr-alpha6
• Species: M. musculus (mouse)
• Insert Size (bp): 2921
• GenBank ID: Accession Number, BC031985
• Entrez Gene: Chrna6 (a.k.a. Acra6, Nica6)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry