pCDNA3.1-nAChr-beta3
(Plasmid #48811)

• Purpose: This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
• Depositing Labs: Henry Lester, Jerry Stitzel
• Publication: Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epub 2007 Oct 11.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5597
• Total vector size (bp): 7127
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nAChr Beta 3 subunit, isoform 1
• Species: M. musculus (mouse)
• Insert Size (bp): 1530
• Mutation: *
• GenBank ID: AAL75573.1 AF467896_1
• Entrez Gene: Chrnb3 (a.k.a. 5730417K16Rik, Acrb3)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Compared to the NCBI Genbank Entrez entry for nAChr-beta3, this plasmid has a K107R amino acid residue substitution. See accession ID: AAL75573.1|AF467896_1 for the correct reference sequence.