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Addgene

pLJM60-S6K1(T389S) barcoded
(Plasmid #48800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM1
  • Backbone manufacturer
    Sabatini lab
  • Backbone size w/o insert (bp) 7400
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S6K1 T389S
  • Alt name
    ribosomal protein S6 kinase T389S
  • Alt name
    S6K1
  • Alt name
    ribosomal protein S6 kinase
  • Species
    R. norvegicus (rat)
  • Mutation
    T389S
  • GenBank ID
    AAA42103.1
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • barcode: GGTACC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pLJM1-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM60-S6K1(T389S) barcoded was a gift from David Sabatini (Addgene plasmid # 48800 ; http://n2t.net/addgene:48800 ; RRID:Addgene_48800)

  • For your References section:

    mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, Turk BE, Yaffe MB, Sabatini DM. Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. 10.1126/science.1236566 PubMed 23888043
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