pcDNA3.1-HA-14-3-3 ϵ
(Plasmid #48797)

• Purpose: expresses human myc-14-3-3ϵ in mammalian cells
• Depositing Lab: Huda Zoghbi
• Publication: Chen et al Cell. 2003 May 16;113(4):457-68.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH10B or similar
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YWHAE 14-3-3 ϵ
• Alt name: 14-3-3 ϵ
• Species: H. sapiens (human)
• Insert Size (bp): 789
• Entrez Gene: YWHAE (a.k.a. 14-3-3E, HEL2, KCIP-1, MDCR, MDS)
• Promoter: cmv
• Tag / Fusion Protein:
  • haemagglutinin (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry