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pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ
(Plasmid #48795)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B or similar
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    YWHAE
  • Alt name
    14-3-3ϵ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    789
  • GenBank ID
  • Entrez Gene
    YWHAE (a.k.a. 14-3-3E, HEL2, KCIP-1, MDCR, MDS)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GGAATTCATGGATGATCGAGAGGATCTGGTG
  • 3′ sequencing primer AGAATTCTCAAGCATAATCTGGAACATCATATGGATACTGATTTTCGTCTTCCACGTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    YWHAZ
  • Alt name
    14-3-3 zeta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    738
  • GenBank ID
    NM_001135699.1
  • Entrez Gene
    YWHAZ (a.k.a. 14-3-3-zeta, HEL-S-3, HEL-S-93, HEL4, KCIP-1, POPCHAS, YWHAD)
  • Promoter CMV (IRES)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCTCTAGAATGGATAAAAATGAGCTGGTTCAG
  • 3′ sequencing primer GCTCTAGATTACAGATCCTCTTCTGAGATGAGTTTTTGTTCATTTTCCCCTCCTTCTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ was a gift from Huda Zoghbi (Addgene plasmid # 48795 ; http://n2t.net/addgene:48795 ; RRID:Addgene_48795)

  • For your References section:

    Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1. Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY. Cell. 2003 May 16;113(4):457-68. 10.1016/S0092-8674(03)00349-0 PubMed 12757707
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