YFP-synDAT-S/D
(Plasmid #48793)

• Purpose: Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro
• Depositing Lab: Jonathan Javitch
• Publication: Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Backbone

• Vector backbone: pCIHygro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6516
• Total vector size (bp): 8376
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP-synhDAT-S/D
• Alt name: SLC6A3
• Species: H. sapiens (human)
• Insert Size (bp): 8376
• Mutation: Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simultaneously to aspartates
• Entrez Gene: SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are several mismatches between Addgene's quality control sequence and the reference sequence provided by the depositing lab, most notably a K to Q mutation in the YFP. These changes should not affect plasmid function.