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Addgene

YFP-synDAT-S/A
(Plasmid #48792)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48792 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCIHygro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6516
  • Total vector size (bp) 8376
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-synhDAT-S/A
  • Alt name
    SLC6A3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8376
  • Mutation
    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simultaneously to alanines
  • Entrez Gene
    SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several mismatches between Addgene's quality control sequence and the reference sequence provided by the depositing lab, most notably a K to Q mutation in the YFP. These changes should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-synDAT-S/A was a gift from Jonathan Javitch (Addgene plasmid # 48792 ; http://n2t.net/addgene:48792 ; RRID:Addgene_48792)
  • For your References section:

    Calmodulin kinase II interacts with the dopamine transporter C terminus to regulate amphetamine-induced reverse transport. Fog JU, Khoshbouei H, Holy M, Owens WA, Vaegter CB, Sen N, Nikandrova Y, Bowton E, McMahon DG, Colbran RJ, Daws LC, Sitte HH, Javitch JA, Galli A, Gether U. Neuron. 2006 Aug 17;51(4):417-29. 10.1016/j.neuron.2006.06.028 PubMed 16908408