YFP-synDAT-S/A
(Plasmid
#48792)
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PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIHygro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6516
- Total vector size (bp) 8376
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP-synhDAT-S/A
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Alt nameSLC6A3
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SpeciesH. sapiens (human)
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Insert Size (bp)8376
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MutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simultaneously to alanines
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Entrez GeneSLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are several mismatches between Addgene's quality control sequence and the reference sequence provided by the depositing lab, most notably a K to Q mutation in the YFP. These changes should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-synDAT-S/A was a gift from Jonathan Javitch (Addgene plasmid # 48792 ; http://n2t.net/addgene:48792 ; RRID:Addgene_48792) -
For your References section:
Calmodulin kinase II interacts with the dopamine transporter C terminus to regulate amphetamine-induced reverse transport. Fog JU, Khoshbouei H, Holy M, Owens WA, Vaegter CB, Sen N, Nikandrova Y, Bowton E, McMahon DG, Colbran RJ, Daws LC, Sitte HH, Javitch JA, Galli A, Gether U. Neuron. 2006 Aug 17;51(4):417-29. 10.1016/j.neuron.2006.06.028 PubMed 16908408