pJSM101
(Plasmid
#48791)
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PurposeNHEJ reporter plasmid for 5' and 3' cohesive ends
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48791 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmsg288
- Total vector size (bp) 12252
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsKanamycin 40mcg/ml
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesplit lacZ
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJSM101 was a gift from Michael Glickman (Addgene plasmid # 48791 ; http://n2t.net/addgene:48791 ; RRID:Addgene_48791) -
For your References section:
Characterization of Mycobacterium smegmatis PolD2 and PolD1 as RNA/DNA polymerases homologous to the POL domain of bacterial DNA ligase D. Zhu H, Bhattarai H, Yan HG, Shuman S, Glickman MS. Biochemistry. 2012 Dec 21;51(51):10147-58. doi: 10.1021/bi301202e. Epub 2012 Dec 11. 10.1021/bi301202e PubMed 23198659