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Addgene

pRGM10
(Plasmid #48790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRGM9
  • Vector type
    DSB repair reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use Kanamycin at 40ug/ml and grow cultures for a longer time than normal (approximately 20 hours)
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    lacZ(I-SceI)
  • Insert Size (bp)
    2570

Gene/Insert 2

  • Gene/Insert name
    lacZ-deltaN
  • Insert Size (bp)
    2439

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGM10 was a gift from Michael Glickman (Addgene plasmid # 48790 ; http://n2t.net/addgene:48790 ; RRID:Addgene_48790)

  • For your References section:

    Mycobacteria exploit three genetically distinct DNA double-strand break repair pathways. Gupta R, Barkan D, Redelman-Sidi G, Shuman S, Glickman MS. Mol Microbiol. 2011 Jan;79(2):316-30. doi: 10.1111/j.1365-2958.2010.07463.x. Epub 2010 Nov 24. 10.1111/j.1365-2958.2010.07463.x PubMed 21219454
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