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Addgene

pRGM1
(Plasmid #48789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmsg383
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use Hygromycin at 150ug/ml and grow cultures for a longer time than normal (approximately 20 hours)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    I-SceI
  • Insert Size (bp)
    753
  • Promoter MOP
  • Tag / Fusion Protein
    • HA tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGM1 was a gift from Michael Glickman (Addgene plasmid # 48789 ; http://n2t.net/addgene:48789 ; RRID:Addgene_48789)

  • For your References section:

    Mycobacteria exploit three genetically distinct DNA double-strand break repair pathways. Gupta R, Barkan D, Redelman-Sidi G, Shuman S, Glickman MS. Mol Microbiol. 2011 Jan;79(2):316-30. doi: 10.1111/j.1365-2958.2010.07463.x. Epub 2010 Nov 24. 10.1111/j.1365-2958.2010.07463.x PubMed 21219454
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