pPy-CAG-GFP-IRES-Pac
(Plasmid
#48759)
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PurposeCAG promoter driving GFP-IRES-Puromycin Acetyl Transferase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48759 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 7300
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Vector typeMammalian Expression ; pPy
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesSynthetic
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Insert Size (bp)700
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CAG forward
- 3′ sequencing primer pA backward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPy-CAG-GFP-IRES-Pac was a gift from Austin Smith (Addgene plasmid # 48759 ; http://n2t.net/addgene:48759 ; RRID:Addgene_48759) -
For your References section:
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A. Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012. 10.1016/j.cell.2013.03.012 PubMed 23582324
Map uploaded by the depositor.
