pPB-CAG-3xFLAG::Fnip1-pgk-hph
(Plasmid
#48758)
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PurposePiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48758 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript
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Vector typeMammalian Expression ; PiggyBac
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xFLAG::Fnip1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3500
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GenBank IDNM_173753
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Entrez GeneFnip1 (a.k.a. A730024A03Rik)
- Promoter CAG
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CAG forward
- 3′ sequencing primer pA backward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-3xFLAG::Fnip1-pgk-hph was a gift from Austin Smith (Addgene plasmid # 48758 ; http://n2t.net/addgene:48758 ; RRID:Addgene_48758) -
For your References section:
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A. Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012. 10.1016/j.cell.2013.03.012 PubMed 23582324