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Purpose(Empty Backbone) PiggyBac backbone, CAG promoter, Hygromycin selection
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBluescript
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Vector typeMammalian Expression ; PiggyBac
- Promoter CAG
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid map is theoretical and may not represent actual sequence. The presence of important plasmid features have been verified by sequencing.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-empty-pgk-hph was a gift from Austin Smith (Addgene plasmid # 48753 ; http://n2t.net/addgene:48753 ; RRID:Addgene_48753) -
For your References section:
Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A. Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012. 10.1016/j.cell.2013.03.012 PubMed 23582324
Map uploaded by the depositor.
