pCMV10-Flag-Fbxl3
(Plasmid
#48751)
-
PurposeExpression of FLAG-tagged Fbxl3
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 48751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonep3xFLAG-CMV-10
-
Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6299
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFbxl3
-
SpeciesM. musculus (mouse)
-
Entrez GeneFbxl3 (a.k.a. AU041772, AW212966, FBK, Fbl3a, Fbxl3a, Ovtm, Play68)
-
Tag
/ Fusion Protein
- 3XFLAG (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV10-Flag-Fbxl3 was a gift from Joseph Takahashi (Addgene plasmid # 48751 ; http://n2t.net/addgene:48751 ; RRID:Addgene_48751) -
For your References section:
Circadian mutant Overtime reveals F-box protein FBXL3 regulation of cryptochrome and period gene expression. Siepka SM, Yoo SH, Park J, Song W, Kumar V, Hu Y, Lee C, Takahashi JS. Cell. 2007 Jun 1;129(5):1011-23. Epub 2007 Apr 26. 10.1016/j.cell.2007.04.030 PubMed 17462724