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Addgene

pET-UNC60A
(Plasmid #48739)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-3a
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C. elegans UNC-60A cDNA
  • Alt name
    C. elegans ADF/cofilin
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    495
  • Entrez Gene
    unc-60 (a.k.a. CELE_C38C3.5)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-UNC60A was a gift from Shoichiro Ono (Addgene plasmid # 48739 ; http://n2t.net/addgene:48739 ; RRID:Addgene_48739)

  • For your References section:

    Two Caenorhabditis elegans actin depolymerizing factor/cofilin proteins, encoded by the unc-60 gene, differentially regulate actin filament dynamics. Ono S, Benian GM. J Biol Chem. 1998 Feb 6;273(6):3778-83. 10.1074/jbc.273.6.3778 PubMed 9452511
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