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Addgene

pFRT-TODestFLAGHAhTTP
(Plasmid #48738)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48738 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl Lab
  • Backbone size w/o insert (bp) 5373
  • Total vector size (bp) 6386
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TTP
  • Alt name
    ZFP36
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1013
  • GenBank ID
    NM_003407
  • Entrez Gene
    ZFP36 (a.k.a. G0S24, GOS24, NUP475, RNF162A, TIS11, TTP, zfp-36)
  • Promoter CMV, 2x Tet
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TODestFLAGHAhTTP was a gift from Thomas Tuschl (Addgene plasmid # 48738 ; http://n2t.net/addgene:48738 ; RRID:Addgene_48738)
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