pRwB
(Plasmid #48734)

• Purpose: dsRed plasmid
• Depositing Lab: John Schiller
• Publication: Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 2008 Dec 10.

Backbone

• Vector backbone: custom
• Total vector size (bp): 5787
• Vector type: Mammalian Expression
• Selectable markers: Zeocin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dsRed-Express
• Species: Discosoma sp
• Promoter: EF-1a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this plasmid is substantially smaller than the ~8kb native papillomavirus genome. There is a second version of this plasmid with a 2kb stuffer region that is available here: http://www.addgene.org/48733 . Further information can be found here: http://home.ccr.cancer.gov/Lco/target.htm