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pTYB1-pLAC-N71
(Plasmid #48720)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB1
  • Backbone manufacturer
    New England BioLabs, INC
  • Backbone size w/o insert (bp) 7477
  • Total vector size (bp) 147
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ER2566
  • Growth instructions
    Bacterial Strain was selected due to its ability to efficiently express mammalian protein (non-glycosolated) in bacterial cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lacritin
  • Alt name
    LACRT
  • Alt name
    pLAC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    147
  • Mutation
    Deleted first 71 amino acids of mature lacritin without signal peptide
  • GenBank ID
    NM_033277.1
  • Entrez Gene
    LACRT
  • Promoter T7
  • Tag / Fusion Protein
    • Intein (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer ATGAAGTATCTTACTAAC
  • 3′ sequencing primer TCATGCCCATGGTTTTAA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that amino acid numbering of this plasmid is relative to amino acid #20 in GenBank reference sequence NP_150593.1. E20 in the reference sequence is considered the first amino acid of lacritin in this plasmid.

Lacritin protein sequence in this plasmid is: SILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTYB1-pLAC-N71 was a gift from Gordon Laurie (Addgene plasmid # 48720 ; http://n2t.net/addgene:48720 ; RRID:Addgene_48720)

  • For your References section:

    Lacritin rescues stressed epithelia via rapid forkhead box O3 (FOXO3)-associated autophagy that restores metabolism. Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW. J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 10.1074/jbc.M112.436584. Epub 2013 May 2. 10.1074/jbc.M112.436584 PubMed 23640897
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