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Addgene

CBIG-Sox5 variant 3
(Plasmid #48707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CBIG
  • Total vector size (bp) 10843
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox5
  • Alt name
    Sox5 variant 3
  • Species
    M. musculus (mouse)
  • Mutation
    variant 3
  • Entrez Gene
    Sox5 (a.k.a. A730017D01Rik)
  • Promoter CMV, beta-actin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence from the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBIG-Sox5 variant 3 was a gift from Jeffrey Macklis (Addgene plasmid # 48707 ; http://n2t.net/addgene:48707 ; RRID:Addgene_48707)
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