5' EGFP SNF-5 pXOON
(Plasmid #48700)

• Purpose: Encodes 5' EGFP - SNF-5 fusion construct for expression in Xenopus oocytes or Mammalian Cells
• Depositing Lab: Dmitri Boudko
• Publication: 5' EGFP SNF-5 pXOON (unpublished)

Backbone

• Vector backbone: pXOON
• Backbone size w/o insert (bp): 5790
• Total vector size (bp): 7977
• Modifications to backbone: EGFP added at 5' end of open reading frame for fusion to snf-5.
• Vector type: Mammalian Expression ; Xenopus Oocyte Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: snf-5
• Alt name: CeNAT5
• Species: C. elegans (nematode)
• Insert Size (bp): 2187
• Entrez Gene: snf-5 (a.k.a. CELE_Y46G5A.30)
• Promoter: T7
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ATG GCC GAT TCG GGC AGC AAT GA
• 3′ sequencing primer: T TAA TAA ACT TGA TGA TAT GTA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original plasmid (pXOON) was donated by the laboratory of Thomas Jespersen at the University of Copanhagen, Denmark.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry