3' EGFP pXOON
(Plasmid #48699)

• Purpose: (Empty Backbone) Creates a 3' Fusion of EGFP to insert for expression in Xenopus oocytes or mammalian cells
• Depositing Lab: Dmitri Boudko
• Publication: EGFP Fuxion pXOON plasmids (unpublished)

Backbone

• Vector backbone: pXOON
• Backbone size (bp): 5775
• Modifications to backbone: EGFP coding sequence added to the 3' end of the MCS for fusion to insert.
• Vector type: Mammalian Expression ; Xenopus Oocyte Expression
• Promoter: T7
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original plasmid (pXOON) was donated by the laboratory of Thomas Jespersen at the University of Copenhagen, Denmark.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry