pFRT-TODestFLAGHAhFMRPiso1I304N
(Plasmid #48692)

• Purpose: Expresses hFMRP iso 1 I304N
• Depositing Lab: Thomas Tuschl
• Publication: Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 2012 Dec 12.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Life Technologies, Modified by Tuschl Lab
• Backbone size w/o insert (bp): 5322
• Total vector size (bp): 7307
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FlagHA FMR1 Iso 1 I304N
• Alt name: FMR1
• Alt name: FMRP
• Alt name: fragile X mental retardation 1
• Species: H. sapiens (human)
• Insert Size (bp): 1985
• GenBank ID: NM_002024
• Entrez Gene: FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
• Promoter: CMV, 2x Tet
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pFRT-TODestFLAGHAhFMRPiso1_I304N.sbd
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry