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pFRT-TODestFLAGHAhFMRPiso1
(Plasmid #48690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl Lab
  • Backbone size w/o insert (bp) 5322
  • Total vector size (bp) 7307
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlagHA FMR1 Iso 1
  • Alt name
    FMR1
  • Alt name
    FMRP
  • Alt name
    fragile X mental retardation 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1985
  • GenBank ID
    NM_002024
  • Entrez Gene
    FMR1 (a.k.a. FMRP, FRAXA, POF, POF1)
  • Promoter CMV, 2x Tet
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TODestFLAGHAhFMRPiso1 was a gift from Thomas Tuschl (Addgene plasmid # 48690 ; http://n2t.net/addgene:48690 ; RRID:Addgene_48690)

  • For your References section:

    FMRP targets distinct mRNA sequence elements to regulate protein expression. Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler U, Tuschl T. Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 2012 Dec 12. 10.1038/nature11737 PubMed 23235829
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