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Purpose(Empty Backbone) 3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUltra-Chili
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Backbone manufacturerMalcolm Moore lab
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Modifications to backboneLuciferase added in XbaI/BamHI sites.
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer DsRed1-C
- 3′ sequencing primer lucNRev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Addgene plasmid #24129 for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUltra-Chili-Luc was a gift from Malcolm Moore (Addgene plasmid # 48688 ; http://n2t.net/addgene:48688 ; RRID:Addgene_48688) -
For your References section:
CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment. Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH. Mol Cancer Ther. 2021 Jun 4. pii: 1535-7163.MCT-20-0591. doi: 10.1158/1535-7163.MCT-20-0591. 10.1158/1535-7163.MCT-20-0591 PubMed 34088832