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Purpose(Empty Backbone) 3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUltra
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Backbone manufacturerMalcolm Moore lab
- Backbone size (bp) 8300
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Modifications to backboneeGFP in pULTRA (Addgene plasmid 24129) replaced with dTomato (not the tandem tomato).
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer DsRed1-C (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Addgene plasmid #24129 for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUltra-Chili was a gift from Malcolm Moore (Addgene plasmid # 48687 ; http://n2t.net/addgene:48687 ; RRID:Addgene_48687)