pLZL2372
(Plasmid
#48683)
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PurposepET29a_HHH CODA HIV integrase with C-terminal HIS tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29a
- Total vector size (bp) 6105
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHIV Integrase
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAuthor of the paper; Xiaojie Qi <[email protected]>
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab recommends bacteria strain Rosetta (DE3)pLysS for protein expression.
Please contact [email protected] for additional information about this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLZL2372 was a gift from Suzanne Sandmeyer (Addgene plasmid # 48683 ; http://n2t.net/addgene:48683 ; RRID:Addgene_48683) -
For your References section:
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. Qi X, Vargas E, Larsen L, Knapp W, Hatfield GW, Lathrop R, Sandmeyer S. PLoS One. 2013 May 17;8(5):e63957. doi: 10.1371/journal.pone.0063957. Print 2013. 10.1371/journal.pone.0063957 PubMed 23691126
