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Addgene

pLZL2386
(Plasmid #48682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Total vector size (bp) 6417
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PFV Integrase
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Author of the paper; "Xiaojie Qi <[email protected]>

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab recommends bacteria strain Rosetta (DE3)pLysS for protein expression.

Please contact [email protected] for additional information about this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZL2386 was a gift from Suzanne Sandmeyer (Addgene plasmid # 48682 ; http://n2t.net/addgene:48682 ; RRID:Addgene_48682)

  • For your References section:

    Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. Qi X, Vargas E, Larsen L, Knapp W, Hatfield GW, Lathrop R, Sandmeyer S. PLoS One. 2013 May 17;8(5):e63957. doi: 10.1371/journal.pone.0063957. Print 2013. 10.1371/journal.pone.0063957 PubMed 23691126
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