EE-SP!gIII
(Plasmid #48667)

• Purpose: Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers
• Depositing Lab: George Church
• Publication: Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.

Backbone

• Vector backbone: colE1-erm
• Total vector size (bp): 7275
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: S. pyogenes
• Insert Size (bp): 4100
• Promoter: proC

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: rrnB-T2-term
• 3′ sequencing primer: StrepPyoCas9-5UTR-F

Gene/Insert 2

• Gene/Insert name: anti-gIII tracrRNA
• Species: Synthetic
• Insert Size (bp): 750
• Promoter: tracrRNA promoter

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: rrnB-T1-term-Rev

Resource Information

• Supplemental Documents:
  • EE-SP!gIII genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry