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PurposeBacterial nuclease-null ST1 Cas9 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecloDF13-aadA
- Total vector size (bp) 6028
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCas9, nuclease-null
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SpeciesS. thermophilus #1
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Insert Size (bp)3360
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MutationD to A, DH to AA, N to A
- Promoter proC
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer rrnB-T2-term
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameST1 tracrRNA
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SpeciesS. thermophilus #1
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Insert Size (bp)200
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DS-ST1casN- was a gift from George Church (Addgene plasmid # 48659 ; http://n2t.net/addgene:48659 ; RRID:Addgene_48659) -
For your References section:
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. 10.1038/nmeth.2681 PubMed 24076762