DS-ST1casN-
(Plasmid #48659)

• Purpose: Bacterial nuclease-null ST1 Cas9 expression
• Depositing Lab: George Church
• Publication: Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.

Backbone

• Vector backbone: cloDF13-aadA
• Total vector size (bp): 6028
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas9, nuclease-null
• Species: S. thermophilus #1
• Insert Size (bp): 3360
• Mutation: D to A, DH to AA, N to A
• Promoter: proC

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: rrnB-T2-term
• 3′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: ST1 tracrRNA
• Species: S. thermophilus #1
• Insert Size (bp): 200

Resource Information

• Supplemental Documents:
  • DS-ST1casN- genbank
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry